<i>In Silico</i> Functional Annotation and Structural Characterization of Hypothetical Proteins in <i>Bacillus paralicheniformis</i> and <i>Bacillus subtilis</i> Isolated from Honey.

ABSTRACT: Bacillus species are ubiquitous and survive in competitive microbial communities under adverse environmental conditions. Bacillus paralicheniformis and Bacillus subtilis obtained from honey revealed a significant proportion of proteins within their genomes as uncharacterized hypothetical proteins (HPs). A total of 1007 HP sequences were evaluated, resulting in the successful annotation of 56 HPs by assigning specific functions to them. A systematic in silico approach, integrating a range of bioinformatics tools and databases to annotate functions, characterize physicochemical properties, determine subcellular localization, and study protein-protein interactions, was used. Homology and de novo models were generated for the HPs, coupled with iterative remod

SUBMITTER: Bin Hafeez A 

PROVIDER: S-EPMC11904672 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature