Photodynamic Inactivation Mediated by Endogenous Porphyrins of <i>Corynebacterium diphtheriae</i> in Planktonic and Biofilm Forms.

ABSTRACT: Photodynamic inactivation (PDI) has emerged as a promising approach to combat bacterial infections by using light activation of photosensitizers to induce microbial death. This study investigated the potential of endogenous porphyrins produced by Corynebacterium diphtheriae as photosensitizers for PDI. Qualitative analysis revealed the presence of porphyrins in all strains studied, with coproporphyrin III predominating. The addition of 5-aminolevulinic acid (ALA) enhanced porphyrin production, as evidenced by increased fluorescence intensity. In addition, high-performance liquid chromatography with diode array and mass spectrometry detection analyses confirmed the presence of coproporphyrin III and protoporphyrin IX in all strains, and the ALA supplementation did not alter the porph

SUBMITTER: Alves GB 

PROVIDER: S-EPMC11904688 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature