Mechanism of salidroside promoting testosterone secretion induced by H₂O₂ in TM3 Leydig cells based on metabolomics and network pharmacology.

ABSTRACT: Oxidative stress-induced damage is a significant contributor to the impairment of Leydig cells in the testes, potentially diminishing the secretion of testosterone and other androgens, thereby resulting in testosterone deficiency. Salidroside, the principal bioactive constituent derived from Rhodiola, exhibits potent antioxidant properties. This study aims to investigate the underlying mechanisms by which salidroside enhances testosterone secretion. The study investigated the oxidative damage in TM3 cells induced by H₂O₂ and demonstrated that salidroside significantly decreased the levels of ROS and MDA, while increasing the levels of testosterone, SOD, GSH. These changes effectively ameliorated oxidative stress, mitigated oxidative damage, protected TM3 cells, and en

SUBMITTER: Wang Z 

PROVIDER: S-EPMC11904911 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature