Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light.

ABSTRACT: Imaging luminescence kinetics is invaluable in many fields, including biology and chemistry. However, the luminescence lifetime of most photo-activated states is in the low ns-µs range and its measurement requires adding costly image intensifiers to cameras to access the fast phenomena present. Here, the Rectified Imaging under Optical Modulation (RIOM) and Heterodyne Imaging under Optical Modulation (HIOM) protocols make this possible with standard low-cost cameras only, even under ambient light. RIOM and HIOM originate from a thorough theoretical analysis, which showed that modulated illumination of any reversibly photoactivable luminophore probed at high frequency generates components of the luminescence response that are detectable at low frequency: RIOM harnesses the mean luminescence

SUBMITTER: Merceron H 

PROVIDER: S-EPMC11904971 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature