Flagellar Assembly Factor FliW2 De-Represses Helicobacter pylori FlaA-Mediated Motility by Allosteric Obstruction of Global Regulator CsrA.

ABSTRACT:

Background

Helicobacter pylori colonizes the human stomach as a dominant member of the gastric microbiota and constitutively expresses flagellar motility for survival. Carbon storage regulator A (CsrA) is a posttranscriptional global regulator and a critical determinant of H. pylori's motility and pathogenicity. The regulation of H. pylori CsrA is still uncertain although in other species CsrA is reported to be antagonized by small RNAs and proteins. In this study, we attempted to unveil how CsrA is regulated and hypothesized that H. pylori CsrA activity is antagonized by a flagellar assembly factor, FliW2, via protein allosteric obstruction.

Materials and methods

Multiple sequence comparisons indicated that, along its length and in contrast to fliW1, the fliW2 of H. pylori

SUBMITTER: Su MS 

PROVIDER: S-EPMC11905337 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature