Identification of <i>Cr</i>DCL1-mediated microRNA biogenesis in green alga <i>Chlamydomonas reinhardtii</i>.

ABSTRACT: In eukaryotes, microRNAs (miRNAs) are generated by Dicer/Dicer-Like (DCL)-mediated cleavage. Previous studies identified three DCL genes (CrDCL1-3) in Chlamydomonas reinhardtii and indicated that CrDCL3 mediated the production of most miRNAs, while CrDCL1 protein was mainly involved in siRNA biogenesis. The role of CrDCL1 in miRNA biogenesis remains unclear. This study constructed a phylogenetic tree, performed structural analyses of Dicer/DCL proteins from multiple species and screened and verified dcl1 and dcl3 mutant strains. Using CC-5325 and dcl3 mutant as control groups, we performed sRNA-sequencing, RT-qPCR, and Northern blot verification experiments on dcl1 mutant to explore the involvement of CrDCL1 in miRNA bio

SUBMITTER: Sun T 

PROVIDER: S-EPMC11905391 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature