Identification of M2 macrophage markers for predicting outcome and therapeutic response in osteosarcoma: Integrated analysis of single-cell and bulk RNA-sequencing.

ABSTRACT: Identification of effective biomarkers is crucial to improve the efficacy of immunotherapy in patients with osteosarcoma. Tumor-associated M2 macrophages, an important immune cell type in the tumor immune microenvironment, are closely related to the formation and progression of tumors. However, the relationships of M2 macrophages and prognosis and the immunotherapy response to osteosarcoma remain unclear. In this study, we obtained single-cell RNA sequencing (scRNA-seq) data of osteosarcoma from the gene expression omnibus (GEO) database and performed trajectory analysis and cell communication analysis. We then identified M2 macrophage marker genes based on scRNA-seq data of osteosarcoma, and constructed a risk-score model using these genes. Next, we compared the survival status and immune

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11905420 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature