Hypoxemia exerts detrimental effects on the choroid plexuses and cerebrospinal fluid system in rats.

ABSTRACT:

Background

Hypoxemia can cause secondary acute brain injury, but the mechanisms behind it are not entirely clear and could involve disturbances in the brain extracellular fluids. We aimed to explore the effects of hypoxemia on the choroid plexus (CPs) and cerebrospinal fluid (CSF) system in rats.

Methods

Male Sprague Dawley rats were kept in O₂ control in vivo cabinet with either 21% (normoxia) or 8% O₂ (hypoxemia) for up to 48 h. In some cases, signaling of selected cytokines was inhibited prior to hypoxemia. CSF and blood samples were collected by Cisterna Magna puncture and through venous catheters, respectively. The percentages of dead cells in the CPs and ependymal layers (EL) after hypoxemia or normoxia was estimated using TUNEL staining. CP's ult

SUBMITTER: Barakat R 

PROVIDER: S-EPMC11905537 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature