Integrative analysis of serum proteomics and transcriptomics in hepatitis C.

ABSTRACT:

Object

Hepatitis C is a contagious disease caused by infection with the hepatitis C virus (HCV) through blood and mother-to-child routes. This study intends to characterize the serum molecular features of hepatitis C using proteomics and transcriptomics.

Methods

Ctrl (normal population), HCV (population with previous HCV infection), and chronic HCV (patients with persistent HCV infection) groups were set up, and the expression profiles of the proteomes and transcriptomes of serum samples were identified using TMT and RNA-seq. Bioinformatics was applied to perform enrichment analysis and PPI network construction of differentially expressed proteins/genes (DEPs/DEGs). RT-qPCR and western blot verified the expression differences of DEPs/DEGs.

Results

Compared to the Ctrl

SUBMITTER: Wang J 

PROVIDER: S-EPMC11905632 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature