Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome.

ABSTRACT: Dietary protein absorption in neonatal mammals and fishes relies on the function of a specialized and conserved population of highly absorptive lysosome-rich enterocytes (LREs). The gut microbiome has been shown to enhance absorption of nutrients, such as lipids, by intestinal epithelial cells. However, whether protein absorption is also affected by the gut microbiome is poorly understood. Here, we investigate connections between protein absorption and microbes in the zebrafish gut. Using live microscopy-based quantitative assays, we find that microbes slow the pace of protein uptake and degradation in LREs. While microbes do not affect the number of absorbing LRE cells, microbes lower the expression of endocytic and protein digestion machinery in LREs. Using transgene-assisted cell isolat

SUBMITTER: Childers L 

PROVIDER: S-EPMC11906160 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature