Reduction in cardiolipin reduces expression of creatine transporter-1 and creatine transport in growing hCMEC/D3 human brain microvessel endothelial cells.

ABSTRACT: The phospholipid cardiolipin (CL) regulates mitochondrial energy production. Endothelial cells of the blood-brain barrier (BBB) play a vital role in uptake of metabolites into the brain and are enriched in mitochondria. We examined how deficiency in BBB endothelial cell CL regulates the expression of selected drug and metabolite transporters and their function. Cardiolipin synthase-1 (hCLS1) was knocked down in a human brain microvessel endothelial cell line, hCMEC/D3, and CL levels and the mRNA expression of selected BBB drug and metabolite transporters examined. Mock transfected hCMEC/D3 cells served as controls. Incorporation of (¹⁴C)creatine and (¹⁴C)oleate into hCMEC/D3 cells was determined as a measure of solute metabolite transport. In addition, protein express

SUBMITTER: Miller DW 

PROVIDER: S-EPMC11906176 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature