Overview of distinct 8-oxoguanine profiles of messenger RNA in normal and senescent cancer cells.

ABSTRACT:

Background

Cellular senescence plays a key role in the development of cancer, but the underlying mechanisms are unknown. Recently, several recent studies have shown that RNA methylation is closely related to cancer cell aging. 8-Oxoguanine (o⁸G) is an important and widely distributed methylation modification whose role in cancer cell senescence is far from elucidated.

Methods

In this study, senescent cancer cell models (CaCO₂ cells) were constructed by knocking down the ADAR1 gene. RNA immunoprecipitation sequencing was used to identify the o⁸G peaks on messenger RNA (mRNA) of normal CaCO₂ cells and senescent CaCO₂ cells, and the distribution characteristics of mRNA o⁸G modification were identified. Further bi

SUBMITTER: Huang J 

PROVIDER: S-EPMC11906479 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature