Simultaneous isolation and culture of endothelial colony-forming cells, endothelial cells and vascular smooth muscle cells from human umbilical cords.

ABSTRACT:

Background

Regulation of the human umbilical circulation under physiological and pathological conditions remains poorly understood. We previously demonstrated that intrauterine growth restriction (IUGR) is associated with sex-specific alterations in the human umbilical circulation. Our data strongly suggest a differential contribution of subcellular compartmentation depending on fetal sex, vessel type and the presence of IUGR. We therefore developed a protocol to isolate and culture umbilical vascular cells to further investigate the relative contribution of each cell type and subcellular compartmentation to the human umbilical circulation regulation.

Methods and results

Human umbilical cords and cord blood were collected just after delivery. Mononuclear cells were recovered

SUBMITTER: Burger ML 

PROVIDER: S-EPMC11906538 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature