Exogenous mitochondria added on benefits for cellular prion protein overexpression in adipose-derived mesenchymal stem cells treatment on intracranial hemorrhage rat.

ABSTRACT: We examined whether combined exogenous mitochondria (Ex^Mito) and cellular prion protein overexpression (Ove-PrP^C) in adipose-derived mesenchymal stem cell (Ove-PrP^C in ADMSCs) therapy is superior to a single therapy for protecting the brain against intracranial hemorrhage (ICH) in rats. In vitro, compared with the control group, Ex^Mito transfusion into recipient cells (i.e., N2a cells) significantly increased under hypoxic conditions (P < 0.001) and augmented ρ0 cell proliferation and cell-cycle activation (P < 0.001). PrP^C-OE in ADMSCs exhibited higher resistance to H₂O₂-induced cell senescence and mitochondrial and DNA damage compared to ADMSCs (P < 0.001). Rats were categorized into group 1 (sham-control), 2 (I

SUBMITTER: Lin KC 

PROVIDER: S-EPMC11906555 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature