T cell receptor usage and epitope specificity amongst CD8⁺ and CD4⁺ SARS-CoV-2-specific T cells.

ABSTRACT:

Introduction

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has highlighted the critical importance of understanding protective long-lasting immune responses. This study investigates the epitope specificity, T cell receptor (TCR) usage, and phenotypic changes in SARS-CoV-2-specfic CD8⁺ and CD4⁺ T cells over time in convalescent individuals with COVID-19.

Methods

Peripheral blood mononuclear cells (PBMCs) were collected from 28 unvaccinated individuals with primary SARS-CoV-2 infection (6 identified as the D614G variant, clade 20C) and analyzed up to 12 months post-symptom onset. Antigen-specific CD8⁺ and CD4⁺ T cells were analyzed using flow cytometry and si

SUBMITTER: Fahnoe U 

PROVIDER: S-EPMC11906682 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature