Endothelial cell-modified BMSC-GT/PCL nanofiber membrane sheet constructs promote bone tissue regeneration.

ABSTRACT:

Introduction

Bone defect repair remains a major challenge in modern medicine. Although bone marrow mesenchymal stem cells (BMSCs) possess multilineage differentiation potential, traditional BMSC constructs are often limited in clinical applications due to insufficient osteogenic differentiation efficiency and inadequate vascularization.

Methods

This study developed an innovative bone tissue engineering strategy by combining BMSCs with gelatin/polycaprolactone (GT/PCL) nanofiber membranes to form cell sheets, which were then modified with endothelial cells (ECs) on the surface. The sheets were subsequently rolled into three-dimensional scaffolds to systematically evaluate their osteogenic potential and underlying mechanisms.

Resuilts

Results showed that electrospun GT/

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC11906688 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature