Typing of <i>Echinococcus multilocularis</i> by Region-Specific Extraction and Next-Generation Sequencing of the mitogenome.

ABSTRACT:

Background

Infection by the fox tapeworm Echinococcus multilocularis may lead to a severe zoonosis in humans, alveolar echinococcosis, which may be fatal if left untreated. Typing is important to understand the epidemiology of this parasite, yet there is limited knowledge on the microdiversity of E. multilocularis on the local scale, since the typing resolution of established methods is restricted.

Methods

The mitogenome of E. multilocularis was used as the target regions to modify, apply and validate the Region-Specific Extraction (RSE) method in combination with Next-Generation Sequencing (NGS). Single Nucleotide Polymorphisms (SNPs) were detected in the mitochondrial DNA (mtDNA) and analysed bioinformatically. To validate the success and the accuracy

SUBMITTER: Rachel F 

PROVIDER: S-EPMC11906691 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature