Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis.

ABSTRACT: Critical to the mechano-regulation of mesenchymal stem cells (MSC), Linker of the Nucleoskeleton and Cytoskeleton (LINC) complex transduces cytoskeletal forces to the nuclei. The LINC complex contains outer nuclear membrane Nesprin proteins that associate with the cytoskeleton and their inner nuclear membrane couplers, SUN proteins. Here we tested the hypothesis that severing of the LINC complex-mediated cytoskeletal connections may have different effects on chromatin organization and MSC differentiation than those due to ablation of SUN proteins. In cells cultured under adipogenic conditions, interrupting LINC complex function through dominant-negative KASH domain expression (dnKASH) increased adipogesis while heterochromatin H3K27 and H3K9 methylation was unaltered. In contrast, SUN1/2 d

SUBMITTER: Goelzer M 

PROVIDER: S-EPMC11906923 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature