Single-cell spatial transcriptomics unravels cell states and ecosystems associated with clinical response to immunotherapy.

ABSTRACT:

Background

The tumor microenvironment (TME) is a complex and dynamic ecosystem that is known to influence responses to immunotherapy. We leveraged single-cell spatial transcriptomics to systematically dissect the intricate complexity of the TME, in particular the cellular heterogeneity and spatial interactions. Their collective impact on immunotherapy efficacy was studied in the context of a homogeneous group of patients with vulvar high-grade squamous intraepithelial lesions (vHSIL) treated with an immunotherapeutic tumor-specific peptide vaccine.

Methods

We performed single-cell spatial transcriptomics on 20 pretreatment vHSIL lesions, stratified by clinical response to immunotherapeutic vaccination into complete responders (CR), partial responders (PR) and non-responders

SUBMITTER: Abdulrahman Z 

PROVIDER: S-EPMC11907085 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature