Transcriptomic insights into developmental arrest in fluorescent labeling transgenic Asian elephant (<i>Elephas maximus</i>) embryos via inter-order cloning.

ABSTRACT:

Introduction

Asian elephants (Elephas maximus) provide a unique model for studying cloning in large mammals. As an endangered species with declining populations and limited oocyte availability, interspecies somatic cell nuclear transfer (iSCNT) combined with transcriptomic analysis holds promise for advancing iSCNT embryonic arrest development and further facilitating applications in conservation efforts, therapeutic cloning, and regenerative medicine.

Methods

This study conducted low-input RNA sequencing analyses on transgenic Asian elephant-pig (AE-P) inter-order cloned embryos expressing enhanced green fluorescent protein (EGFP) at the 2- and 4-cell stages. Differential gene expressions, pathway enrichment, and hub gene analyses were performed to identify the molec

SUBMITTER: Pankammoon P 

PROVIDER: S-EPMC11907086 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature