Genetically stable multi-gene edited iPSCs-derived NK cells for enhanced cancer immunotherapy.

ABSTRACT: Chimeric antigen receptor (CAR)-T cell treatment is an innovative drug with excellent therapeutic effects against B cell blood cancer. However, multiple side effects and ultra-high treatment costs must be overcome. Off-the-shelf CAR natural killer (NK) cells can be a good alternative to patient-specific CAR-T cells. The purpose of this study was to combine cellular reprogramming, gene editing, and differentiation technologies to produce full-off-the-shelf NK cells and to verify their efficacy and safety. Genetically stable universal and potent CAR (upCAR)-induced pluripotent stem cells (iPSCs) showed biallelic insertions and deletions in the coding sequence and no off-target effects. upCAR-NK cells showed a very high differentiation yield and in vitro proliferation, and freezing/tha

SUBMITTER: Kwon D 

PROVIDER: S-EPMC11907209 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature