MiR-451a attenuates hepatic steatosis and hepatitis C virus replication by targeting glycerol kinase.

ABSTRACT:

Background

Lipotoxicity is one of the causes for the progression of fatty liver in chronic hepatitis (CH) towards end-stage liver diseases. The role of miRNAs in the signalling pathways of lipid metabolism has been studied, but their direct targets in this pathway have not been identified yet. Here, we have characterized a downregulated miRNA in CH namely miR-451a, which has a direct impact on the lipid metabolism pathway.

Methods

Liver tissue samples and blood were collected from CHC/CHB patients and normal individuals. Huh7 and SNU449 cell lines were used for in vitro assays. Expressions of miRNA/mRNAs and proteins were confirmed by qRT-PCR and immuno-blot analysis. Oil Red O staining, Colorimetric, and Fluorometric assay kit were used to quantify triglyceride (TG) and cho

SUBMITTER: Majumdar S 

PROVIDER: S-EPMC11907786 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature