Genome-wide screening in human embryonic stem cells identifies genes and pathways involved in the p53 pathway.

ABSTRACT:

Background

The tumor suppressor protein, p53, which is mutated in half of human tumors, plays a critical role in cellular responses to DNA damage and maintenance of genome stability. Therefore, increasing our understanding of the p53 pathway is essential for improving cancer treatment and diagnosis.

Methods

This study, which aimed to identify genes and pathways that mediate resistance to p53 upregulation, used genome-wide CRISPR-Cas9 loss-of-function screening done with Nutlin-3a, which inhibits p53-MDM2 interaction, resulting in p53 accumulation and apoptotic cell death. We used bioinformatics analysis for the identification of genes and pathways that are involved in the p53 pathway and cell survival assays to validate specific genes. In addition, we used RNA-seq to identif

SUBMITTER: Haddad A 

PROVIDER: S-EPMC11907909 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature