Bacterial translocation and gut microbiome imbalance in an experimental infection model of legionellosis in guinea pigs.

ABSTRACT:

Background

Recent studies have shown that in critically ill patients such as those with sepsis and shock, the lung and gut microbiomes undergo profound changes. Legionella pneumophila (Lp) can cause fatal infection, however, such changes have not been investigated in legionellosis. Here, we evaluated the microbiome of the lungs, blood, liver, and small intestine content in Lp-infected guinea pigs.

Methods

We used a culture-independent method by analysing the conserved 16S rDNA sequences of bacteria from the organs of guinea pigs infected with legionellosis. Bacterial DNA was also identified through bacterial probe-fluorescence in situ hybridisation (BP-FISH). Bacterial entry from the intestinal lumen into the submucosa was examined via ultrastructural visualisation.

Resul

SUBMITTER: Cai X 

PROVIDER: S-EPMC11908093 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature