Enantioselective synthesis of (<i>R</i>)-citronellal from geraniol with an immobilised copper alcohol oxidase and ene reductase.

ABSTRACT: (R)-Citronellal is one of the key chiral intermediates in the synthesis of the isomer (-)-menthol, one of the most commercialised terpenoid flavours worldwide. Enzymatic approaches could represent a less energy-demanding alternative for its synthesis, such as a previously reported bienzymatic cascade starting from inexpensive, commercially available geraniol. A copper radical oxidase (CgrAlcOx) followed by a flavin-dependent ene reductase (OYE2) were used to obtain (R)-citronellal. Here, we used a metal-affinity immobilisation strategy on the His-tagged enzymes for the cascade and studied enzyme recovery and reusability as well as increased solvent tolerance. After screening a panel of resins for enzyme immobilisation and water-immiscible co-solvents, we successfully o

SUBMITTER: Tagliabue B 

PROVIDER: S-EPMC11908116 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature