Hierarchical Lineage Tracing Reveals Diverse Pathways of AML Treatment Resistance.

ABSTRACT: Cancer cells adapt to treatment, leading to the emergence of clones that are more aggressive and resistant to anti-cancer therapies. We have a limited understanding of the development of treatment resistance as we lack technologies to map the evolution of cancer under the selective pressure of treatment. To address this, we developed a hierarchical, dynamic lineage tracing method called FLARE (Following Lineage Adaptation and Resistance Evolution). We use this technique to track the progression of acute myeloid leukemia (AML) cell lines through exposure to Cytarabine (AraC), a front-line treatment in AML, in vitro and in vivo. We map distinct cellular lineages in murine and human AML cell lines predisposed to AraC persistence and/or resistance via the upregulation of cell adhesion and moti

SUBMITTER: Saxe R 

PROVIDER: S-EPMC11908168 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature