AI-Driven Analysis Unveils Functional Dynamics of Muller Cells in Retinal Autoimmune Inflammation.

ABSTRACT: Müller cells are the most abundant glial cell type in the human and mouse retina, playing a crucial role in maintaining retinal homeostasis. However, many aspects of Müller cell function remain poorly characterized. In this study, we reanalyzed a single-cell RNA-seq (scRNA-seq) dataset from Aire-/- mice, focusing on Müller cells and T cells. We identified nine distinct Müller cell subgroups and five T cell subgroups, with activated Müller cells comprising the majority of the Müller cells in the inflamed retina. Using SCassist, an AI-based workflow assistant for single-cell analysis, we created a comparison matrix to quantify pathway involvement in each Müller cell subset. This approach unveils the functional dynamics of Müller cells during retinal inflammation. Activated Müller cells prima

SUBMITTER: Shi G 

PROVIDER: S-EPMC11908203 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature