Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy.

ABSTRACT: Recombinant proteins are critical for modern therapeutics and diagnostics, with Chinese hamster ovary (CHO) cells serving as the primary production platform. However, environmental and chemical stressors in bioreactors often trigger cell death, particularly apoptosis, posing a significant challenge to recombinant protein manufacturing. Rapid, label-free methods to monitor cell death are essential for ensuring better production quality. Stimulated Raman scattering (SRS) microscopy offers a powerful, label-free approach to measure lipid and protein compositions in live cells. We demonstrate that SRS microscopy enables rapid and reagent-free analysis of apoptotic and necrotic transitions. Our results show that apoptotic cells exhibit higher protein concentrations, while necrotic cells show an

SUBMITTER: Mahapatra S 

PROVIDER: S-EPMC11908225 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature