The KASH protein UNC-83 differentially regulates kinesin-1 activity to control developmental stage-specific nuclear migration.

ABSTRACT: Nuclear migration plays a fundamental role in development, requiring precise spatiotemporal control of bidirectional movement through dynein and kinesin motors. Here, we uncover a mechanism for developmental regulation of nuclear migration directionality. The nuclear envelope KASH protein UNC-83 in Caenorhabditis elegans exists in multiple isoforms that differentially control motor activity. The shorter UNC-83c isoform promotes kinesin-1-dependent nuclear movement in embryonic hyp7 precursors, while longer UNC-83a/b isoforms facilitate dynein-mediated nuclear migration in larval P cells. We demonstrate that UNC-83a's N-terminal domain functions as a kinesin-1 inhibitory module by directly binding kinesin heavy chain (UNC-116). This isoform-specific inhibition, combined with differen

SUBMITTER: Gumusderelioglu S 

PROVIDER: S-EPMC11908248 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature