Nucleotide motif-guided selection of plasma microRNA biomarkers for organ injury prediction in trauma.

ABSTRACT:

Background

Trauma remains a leading cause of morbidity and mortality in part due to secondary organ injury and infection. Yet, our ability to predict the downstream pathophysiologic responses leading to organ injury and adverse outcomes is limited. Extracellular microRNAs (ex-miRNAs) as a DAMP can drive innate immune response and organ injury. Here, we tested plasma miRNAs as predictive biomarkers for organ injury in trauma.

Methods

Twelve miRNAs were selected based on RNAseq and pro-inflammatory nucleotide motifs identified by machine learning. Digital PCR was employed to quantify plasma miRNAs and Luminex to measure trauma injury markers. Multivariate Random Forest models were built to assess the predictive performance of the miRNA biomarkers.

Results

We identified

SUBMITTER: Ren B 

PROVIDER: S-EPMC11908285 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature