Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15-25 A.

ABSTRACT: Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.

SUBMITTER: Girvan P 

PROVIDER: S-EPMC11908412 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature