SplintR ligation-triggered <i>in-situ</i> rolling circle amplification on magnetic bead for accurate detection of circulating microRNAs.

ABSTRACT: The circulating microRNAs (miRNAs), endogenous noncoding RNAs, post-transcriptionally participate in multiple processes during cell growth and development. Moreover, dysregulation of miRNAs expression is intricately associated with cancer. Currently, challenges of high homology, sequence similarity, and low abundance encountered in the detection of target miRNAs in complex samples need to be addressed. Biosensors established for miRNAs detection suffer from limitations in terms of sensitivity, specificity and high cost. Herein, a miRNA detection method based on in-situ RCA on magnetic bead catalyzed by SplintR ligase was proposed to achieve high sensitivity and high specificity. The following steps are included: (1) formation of P1-P2-miRNA double-stranded complex under catalyzation

SUBMITTER: Yang S 

PROVIDER: S-EPMC11908441 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature