Modulation of cardiomyocyte contractility and action potentials with chemogenetic chloride currents.

ABSTRACT: Transient perturbation of electrical activity is used in neuroscience to study the impact of specific neuronal cell populations on brain function. Similarly, cardiomyocyte (CM) physiology can be controlled by the activation of artificially expressed ion channels. Pharmacologically selective actuator modules (PSAMs) are engineered ligand-gated ion channels that can be activated with small molecules. We aimed to use the 'inhibitory' PSAMs, (i) PSAM^{L141F,Y115F-GlyR} (PSAM-GlyR) and (ii) PSAM^{L131G,Q139L,Y217F} (ultrapotent PSAM⁴-GlyR), which consist of modified α7-nicotinergic acetylcholine receptor ligand binding domains and the ion pore domain of the glycine receptor, to modulate CM physiology with chloride currents. We employed CRISPR/Cas9 to integrate PSAM-Gl

SUBMITTER: Sonmez M 

PROVIDER: S-EPMC11908473 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature