Identification of positions in human aldolase a that are neutral for apparent K_M.

ABSTRACT: According to evolutionary theory, many naturally-occurring amino acid substitutions are expected to be neutral or near-neutral, with little effect on protein structure or function. Accordingly, most changes observed in human exomes are also expected to be neutral. As such, accurate algorithms for identifying medically-relevant changes must discriminate rare, non-neutral substitutions against a background of neutral substitutions. However, due to historical biases in biochemical experiments, the data available to train and validate prediction algorithms mostly contains non-neutral substitutions, with few examples of neutral substitutions. Thus, available training sets have the opposite composition of the desired test sets. Towards improving a dataset of these critical negative controls, we

SUBMITTER: Swint-Kruse L 

PROVIDER: S-EPMC11908651 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature