Chromosome-scale and haplotype-resolved genome assembly of <i>Populus trichocarpa</i>.

ABSTRACT: Populus trichocarpa, a pivotal model organism for woody transgenic research, not only garners substantial scientific interest but plays an integral role in forestry economics. Previous genomic assemblies of P. trichocarpa predominantly treated its heterozygous genome as homozygous, thereby neglecting crucial haplotypic diversity. Leveraging the high-fidelity (HiFi) sequencing capabilities of PacBio sequencing and the chromosome conformation capture insights provided by Illumina's Hi-C technique, this study is the first to achieve a near telomere-to-telomere assembly of both paternal and maternal haplotypes in P. trichocarpa. Comparative genomic analysis between these haplotypes has uncovered several allelic variants and pathways critical for trait determination through

SUBMITTER: Gao W 

PROVIDER: S-EPMC11908830 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature