Proteomic Changes Associated With Endogenous FBXW7 Mutations in Moderately Differentiated Endometrial Cancer Cells Include Increased TROP2 and Galectin-3 Levels.

ABSTRACT:

Background

Endometrial cancer (EC) is the fourth most commonly diagnosed cancer among women in the US and the fifth leading cause of cancer death in this population. The FBXW7 tumor suppressor gene is frequently mutated in all molecular subtypes of EC. The encoded protein is part of a ubiquitin ligase complex that targets substrate proteins for ubiquitination and, in most instances, proteasome-mediated degradation.

Aims

The purpose of this investigation was to identify the proteomic changes associated with endogenous FBXW7 mutations in EC.

Materials & methods

Quantitative LC-MS/MS was used to identify significant (p < 0.05) differences in the proteomes and phosphoproteomes of two FBXW7-mutated EC cell lines, HEC-1-B^FBXW7-R367X and JHUEM-1^FBXW7-R505C

SUBMITTER: Urick ME 

PROVIDER: S-EPMC11909011 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature