Lab-on-a-chip device for microfluidic trapping and TIRF imaging of single cells.

ABSTRACT: Total internal reflection fluorescence (TIRF) microscopy is a powerful imaging technique that visualizes the outer surface of specimens in close proximity to a substrate, yielding crucial insights in cell membrane compositions. TIRF plays a key role in single-cell studies but typically requires chemical fixation to ensure direct contact between the cell membrane and substrate, which can compromise cell viability and promote clustering. In this study, we present a microfluidic device with structures designed to trap single yeast cells and fix them in direct contact with the substrate surface to enable TIRF measurements on the cell membrane. The traps are fabricated using two-photon polymerization, allowing high-resolution printing of intricate structures that encapsulate cells in all three

SUBMITTER: Dzikonski D 

PROVIDER: S-EPMC11909039 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature