Overexpression of miR-99a promoted expansion and suppressed differentiation of hematopoietic stem/progenitor cells.

ABSTRACT: MicroRNAs (miRNAs) are a class of non-coding RNAs involved in a variety of pathophysiological processes. We have previously reported that the abnormally high expression of miR-99a is associated with drug resistance and poor prognosis in acute myeloid leukemia. However, the impact of miR-99a on normal hematopoiesis is not well understood. To investigate the effect of aberrant miR-99a overexpression on hematopoietic stem and progenitor cells (HSPCs), we overexpressed miR-99a in human umbilical cord blood CD34⁺ cells. We observed that miR-99a overexpression increased the proliferation, self-renewal capacity, and transplantation efficiency of HSPCs with or without a clonal hematopoiesis-associated mutation (JAK2^V617F). Meanwhile, we found that overexpression of miR-99a bl

SUBMITTER: Wang W 

PROVIDER: S-EPMC11909132 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature