A novel strategy for controllable electrofabrication of molecularly imprinted polymer biosensors utilizing embedded Prussian blue nanoparticles.

ABSTRACT: The reproducibility of ultrasensitive biosensors is vital for clinical research, scalable manufacturing, commercialization, and reliable clinical decision-making, as batch-to-batch variations introduce significant uncertainty. However, most biosensors lack robust quality control (QC) measures. This study introduces an innovative QC strategy to produce highly reproducible molecularly imprinted polymer (MIP) biosensors by leveraging real-time data from the electrofabrication process. Prussian Blue nanoparticles (PB NPs) embedded within the MIP structure enable precise monitoring of surface properties, conductivity, MIP film thickness, and template extraction efficiency. The QC strategy utilizes variations in the current intensity of PB NPs during fabrication to implement real-time, non-destr

SUBMITTER: Babamiri B 

PROVIDER: S-EPMC11909151 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature