An automated high-throughput lighting system for screening photosynthetic microorganisms in plate-based formats.

ABSTRACT: The capacity of photosynthetic microorganisms to fix carbon dioxide into biomass positions them as promising cell factories for sustainable biomanufacturing. However, limitations in screening throughput hinder the identification of enzymes, strains, and growth conditions needed to realize this potential. Here we present a microplate-based high-throughput cultivation system that can be integrated into existing automation infrastructure and supports growth of both prokaryotic and eukaryotic photosynthetic microorganisms. We validate this system by optimizing BG-11 medium compositions for Synechococcus elongatus UTEX 2973, Chlamydomonas reinhardtii UTEX 90 and Nostoc hatei CUBC1040, resulting in growth rates increases of 38.4% to 61.6%. We also identify small molecules that influence growth r

SUBMITTER: Noonan AJC 

PROVIDER: S-EPMC11909208 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature