Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells.

ABSTRACT:

Introduction

Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers.

Materials & methods

A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC.

Results

Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 10³ cells/cm² inoculum (i.e. 1 × 10⁶ cells). Cel

SUBMITTER: Franchi-Mendes T 

PROVIDER: S-EPMC11909460 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature