Screening of RNA methyltransferase NSP16 inhibitors against SARS-CoV-2 coronavirus and study of related mechanisms.

ABSTRACT:

Objective

We aimed to determine the abilities of several drugs to block the second methylation process of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA with non-structural protein 16 (NSP16) and expose the virus to the innate immune mechanism of the host for the purpose of improving infection control and drug development for COVID-19.

Methods

Recombinant prokaryotic expression plasmids PET30a-NSP16 and PET15b-NSP10 and a plasmid for preserving the untranslated region (UTR) sequences, pUC57-UTR, were constructed. The obtained UTR template was transcribed in vitro to obtain RNAs. Then, bioluminescence was used to determine the K_m values of NSP16 and non-structural protein 10 (NSP10) and study the inhibition effects of four clinical drugs - cladrib

SUBMITTER: Fan X 

PROVIDER: S-EPMC11909547 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature