Silencing of the von Willebrand factor gene in proatherothrombotic <i>APOE∗3-Leiden.CETP</i> transgenic mice.

ABSTRACT:

Background

Elevated von Willebrand factor (VWF) levels correlate with higher risk of atherosclerosis-related arterial thrombosis (atherothrombosis). Silencing the VWF gene via small-interfering RNAs (siRNAs) could mitigate this risk. Previous studies successfully delivered siRNA to the endothelium of healthy, wild-type (WT) mice using lipid nanoparticles (LNPs).

Objectives

This study aimed to investigate whether the LNP-siRNA strategy could achieve endothelium-specific Vwf-silencing under diseased conditions of prolonged hypercholesterolemia and atherothrombosis-prone vasculature.

Methods

Female transgenic mice expressing a variant of human APOE∗3 (ie, APOE∗3-Leiden) and human cholesteryl ester transfer protein (CETP), fed a choleste

SUBMITTER: Jongejan YK 

PROVIDER: S-EPMC11909755 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature