Proteomics-driven discovery of LCAT as a novel biomarker for liver metastasis in colorectal cancer.

ABSTRACT:

Background

This study aimed to identify molecular markers that influence liver metastasis in colorectal cancer (CRC) and assess their clinical relevance.

Methods

Proteomic analysis compared differential protein expression between CRC patients with liver metastasis (CRLM) and those without (CRNLM). Bioinformatics and survival analyses identified key proteins and validated them using the TCGA database for expression and clinical significance. Clinical and pathological data, along with tissue samples from our center, were used to create tissue microarrays for immunohistochemistry. Logistic regression assessed odds ratios (OR) for molecular markers linked to liver metastasis post-CRC surgery. Stable LCAT knockdown and overexpression CRC cell lines were constructed, and Transwell

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11909834 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature