Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

ABSTRACT:

Background

Cholestatic liver disease, characterized by impaired bile flow, leads to the accumulation of harmful metabolites and toxins, resulting in liver damage. Inflammatory cytokines are crucial for the progression of this condition. Clusterin is a glycoprotein with roles in cell death, lipid transport, and cellular protection. We previously demonstrated that clusterin protects against hepatic steatosis and hepatic fibrosis. This study explored the roles of clusterin in cholestatic liver injury induced by a DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine) diet.

Methods

The study evaluated the impact of clusterin on liver injury in C57BL/6 mice and clusterin-knockout (KO) mice fed a DDC diet for 10-20 days. Primary Kupffer cells (KCs) and hepatocytes (HCs) of these mice wer

SUBMITTER: Seo HY 

PROVIDER: S-EPMC11909925 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature