A novel indicator-based visualisation method to investigate diffusion behaviour of dissolved CO₂ in hydrogels.

ABSTRACT: Biocompatible hydrogels are versatile platforms for encapsulating living cells in biotechnology due to their unique physical, structural and mechanical properties. The diffusion of dissolved carbon dioxide (dCO₂) into the hydrogel matrix is of great importance for the growth of immobilised photosynthetic cells like microalgae and cyanobacteria. However, non-invasive analysis methods for measuring the diffusion of dCO₂ in hydrogels are limited. In this article, we describe an indirect method for the non-invasive measurement of diffusion rates for dCO₂ in hydrogels. We visually tracked the diffusion along the axial direction of pH indicator-doped hydrogel monoliths by recording the interface position over time. We calculated the interface velocity and the pse

SUBMITTER: Fladung L 

PROVIDER: S-EPMC11910120 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature