Protein thermal stability in the undergraduate biochemistry laboratory: Exploring protein thermal stability with yeast alcohol dehydrogenase.

ABSTRACT: We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase (YADH) is used in the undergraduate biochemistry laboratory course at Miami University as the model system to explore protein overexpression and purification, bioinformatics, and enzyme characterization. Using one model protein across the entire semester allows the students to independently link topics introduced in the individual experiments; for example, students might draw connections between the thermal denaturation experiment and the requireme

SUBMITTER: Bates A 

PROVIDER: S-EPMC11910175 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature