Plastidial thioredoxin-like proteins are essential for normal embryogenesis and seed development in Arabidopsis thaliana.

ABSTRACT: Thiol/disulfide-based redox regulation is a key mechanism for modulating protein functions in response to changes in cellular redox status. Two thioredoxin (Trx)-like proteins [atypical Cys His-rich Trx (ACHT) and Trx-like2 (TrxL2)] have been identified as crucial for oxidizing and deactivating several chloroplast enzymes during light-to-dark transitions; however, their roles remain to be fully understood. In this study, we investigated the functions of Trx-like proteins in seed development. Using the CRISPR/Cas9 system, we generated an Arabidopsis quadruple mutant defective in ACHT1, ACHT2, TrxL2.1, and TrxL2.2 (acht/trxl2). This mutant showed increased seed lethality prior to maturation, with embryogenesis impaired primarily during the heart and torpedo stages, which are critical phases

SUBMITTER: Fukushi Y 

PROVIDER: S-EPMC11910432 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature