Caspase 9-induced apoptosis enables efficient fetal cell ablation and disease modeling.

ABSTRACT: Fetal cell ablation models are crucial for studying congenital diseases, organ regeneration, and xenotransplantation. However, conventional knockout models offer limited control over disease severity, while conditional ablation models often require fetus-harming inducers. In the present study, we demonstrate that the inducible caspase 9 system enables precise targeting of fetal nephron progenitor cells in mice through the intrinsic apoptotic pathway. Using a safe, placenta-permeable inducer, this system facilitates specific, rapid, and efficient cell ablation. The system's temporal control allows precise adjustment of disease severity, generating reproducible models ranging from congenital kidney deficiency to severe chronic kidney disease. Cells with low expression levels of inducible cas

SUBMITTER: Matsui K 

PROVIDER: S-EPMC11910536 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature