TRPML1 ion channel promotes HepaRG cell differentiation under simulated microgravity conditions.

ABSTRACT: Stem cell differentiation must be regulated by intricate and complex interactions between cells and their surrounding environment, ensuring normal organ and tissue morphology such as the liver¹. Though it is well acknowledged that microgravity provides necessary mechanical force signals for cell fate², how microgravity affects growth, differentiation, and communication is still largely unknown due to the lack of real experimental scenarios and reproducibility tools. Here, Rotating Flat Chamber (RFC) was used to simulate ground-based microgravity effects to study how microgravity effects affect the differentiation of HepaRG (hepatic progenitor cells) cells. Unexpectedly, the results show that RFC conditions could promote HepaRG cell differentiation which exhibited incr

SUBMITTER: Fan H 

PROVIDER: S-EPMC11910645 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature